Stefan Westermann: A close look at kinetochore function
نویسنده
چکیده
Stefan Westermann is probing the structure and function of the yeast kinetochore.
منابع مشابه
Kinetochores hang on for the ride
C hromosomes are shunted around the cell during mitosis to align at the metaphase plate and then segregate to the spindle poles. These movements are driven by dynamic microtubules, which polymerize and disassemble to pull the chromosomes into position. Chromosomes grab microtubules via their kinetochores, but how do they maintain their grip as the microtubules turn over? “It’s pretty amazing,” ...
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Kinetochores are megadalton-sized protein complexes that mediate chromosome-microtubule interactions in eukaryotes. How kinetochore assembly is triggered specifically on centromeric chromatin is poorly understood. Here we use biochemical reconstitution experiments alongside genetic and structural analysis to delineate the contributions of centromere-associated proteins to kinetochore assembly i...
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Kinetochores are large protein complexes that link sister chromatids to the spindle and transduce microtubule dynamics into chromosome movement. In budding yeast, the kinetochore-microtubule interface is formed by the plus end-associated Dam1 complex and the kinetochore-resident Ndc80 complex, but how they work in combination and whether a physical association between them is critical for chrom...
متن کاملArchitecture of the budding yeast kinetochore reveals a conserved molecular core
How kinetochore proteins are organized to connect chromosomes to spindle microtubules, and whether any structural and organizational themes are common to kinetochores from distantly related organisms, are key unanswered questions. Here, we used affinity chromatography and mass spectrometry to generate a map of kinetochore protein interactions. The budding yeast CENP-C homologue Mif2p specifical...
متن کاملMolecular architecture and connectivity of the budding yeast Mtw1 kinetochore complex.
Kinetochores are large multiprotein complexes that connect centromeres to spindle microtubules in all eukaryotes. Among the biochemically distinct kinetochore complexes, the conserved four-protein Mtw1 complex is a central part of the kinetochore in all organisms. Here we present the biochemical reconstitution and characterization of the budding yeast Mtw1 complex. Direct visualization by elect...
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عنوان ژورنال:
- The Journal of Cell Biology
دوره 179 شماره
صفحات -
تاریخ انتشار 2007